Mueller Hinton Agar I is used for antimicrobial sensitivity testing by the disc diffusion method and is not intended for use in the diagnosis of disease or other conditions in humans. A medium for antimicrobial sensitivity testing by the disc diffusion method. This medium, used in the technique of Bauer and Kirby, has been adopted by the Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS) in the USA as the definitive method for susceptibility testing. The medium has a very low thymine and thymidine content, making it suitable for trimethoprim and sulphonamide testing, controlled to ensure correct zone sizes with aminoglycoside and tetracyline antibiotics. The medium was originally formulated as a heat labile protein free medium for the isolation of pathogenic Neisseriaceae.

• Analyte: E. coli, enterococci, pseudomonas spp., staphylococci
• Platform: dehydrated culture media
• Formula: beef extract 2.0g/L, acid hydrolysed casein 17.5g/L, starch 1.5g/L, agar 17.0g/L
• Grams per litre: 38.00
• Litres per 500g: 13.00
• Plates per 500g: 720.00